You can configure ASP.NET for a Web Forms project before or after you deploy the project to an IIS 5.0 or later server. All files and directories that you access from a Web Forms application must have appropriate ASPNET (IIS 5.0), IIS_WPG (IIS 6.0), or IIS_USRS (IIS 7.0) user permissions.
For an example of granting user permissions to a directory, see “Granting ASP.NET user permissions”.
